PREPARATION OF BIOLOGICALLY DERIVED FLUIDS FOR BIOMARKER DETERMINATION BY MASS SPECTROMETRY

摘要

Methods and kits for preparing biologically derived fluids for subsequent biomarker analysis by mass spectrometry are provided. Using a buffer comprising salt in the range of 0.1% to 2.0%, a chaotropic agent, a reducing agent and a volatile organic acid, the sample is prepared for solid phase adsorption. Thus, the loss of components unbound to the active surface is limited.