摘要 |
PROBLEM TO BE SOLVED: To provide a method by which the polymorphism in a nucleic acid sequence can be detected clearly and in good repeatability; and to obtain a reagent therefor. SOLUTION: The base polymorphism is identified by simultaneously or separately reacting a nucleic acid sequence containing a specific base polymorphism site contained in a sample, with an oligonucleotide for detecting a wild type in which at least one base in bases from the 3' terminus to the fifth base is substituted with a substituent not having interaction with a DNA-type base or an RNA-type base, and one or more kinds of oligonucleotides for detecting a variant. The substituent not having interaction with the DNA-type base or the RNA-type base is preferably inosine. COPYRIGHT: (C)2006,JPO&NCIPI
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