发明名称 |
Method for generating recombinant DNA library using unidirectional single-stranded DNA fragments |
摘要 |
The present invention relates to a method for producing a recombinant polynucleotides comprising the steps of generating a pool of unidirectional single-stranded polynucleotide fragments from two or more homologous double-stranded polynucleotides, conducting a polymerization process comprising multi-cyclic extension reactions using the unidirectional single-stranded polynucleotide fragments as templates and specific oligonucleotides as primers to obtain recombinant polynucleotides, and conducting a polymerase chain reaction using at least one primer to amplify the recombinant polynucleotides; and a method for constructing a recombinant DNA library comprising the steps of inserting the recombinant polynucleotide prepared by the above method into a vector and transforming an expression cell with said vector containing the recombinant polynucleotide to obtain a plurality of mutant clones. The method of the present invention can be used for in vitro recombination of homologous polynucleotides and the directed molecular evolution.
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申请公布号 |
US6955879(B2) |
申请公布日期 |
2005.10.18 |
申请号 |
US20020148724 |
申请日期 |
2002.05.28 |
申请人 |
AMICOGEN, INC. |
发明人 |
LEE SI-HYOUNG;SHIN YONG-CHUL;JEON YEONG-JOONG;JUNG KYUNG-HWA;RYU EUN-JUNG;LEE KO-WOON |
分类号 |
C12N15/09;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C12P21/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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