发明名称 |
A METHOD FOR CHROMATOGRAPHIC PURIFICATION |
摘要 |
<p>The present invention relates to a method of isolating a target compound from other components of a liquid, which method comprises at least two chromatographic steps, in any sequence of order, wherein the mobile phase is contacted with an affinity chromatography matrix and/or an ion-exchange chromatography matrix and/or a hydrophobic interaction chromatography matrix, wherein the contacting with at least one of the matrices takes place in the presence of at least one non-ionic polyether; and obtaining the target compound(s) in a separate fraction from the last chromatographic step. In the most preferred embodiment, the non-ionic polyether is poly(ethylene glycol) (PEG).</p> |
申请公布号 |
WO2005094960(A1) |
申请公布日期 |
2005.10.13 |
申请号 |
WO2005SE00467 |
申请日期 |
2005.03.31 |
申请人 |
AMERSHAM BIOSCIENCES AB;VAN ALSTINE, JAMES;HOUSHMAND, HAMID;LJUNGLOEF, ANDERS;AABERG, PER-MIKAEL |
发明人 |
VAN ALSTINE, JAMES;HOUSHMAND, HAMID;LJUNGLOEF, ANDERS;AABERG, PER-MIKAEL |
分类号 |
B01D;B01D15/08;B01D15/32;B01D15/36;B01D15/38;C07K1/22;C07K1/36;G01N30/46;(IPC1-7):B01D15/08 |
主分类号 |
B01D |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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