发明名称 |
CONTROL OF PLANT GENE EXPRESSION |
摘要 |
A method for making a genetically modified plant comprises regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression result in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also, a genetically modified hybrid plant is made by hybridizing a first plant regenerated from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.
|
申请公布号 |
CA2196410(C) |
申请公布日期 |
2005.10.11 |
申请号 |
CA19952196410 |
申请日期 |
1995.07.31 |
申请人 |
UNITED STATES OF AMERICA, REPRESENTED BY THE SECRETARY OF AGRICULTURE;DELTA AND PINE LAND COMPANY |
发明人 |
OLIVER, MELVIN JOHN;QUISENBERRY, JERRY EDWIN;TROLINDER, NORMA LEE GLOVER;KEIM, DON LEE |
分类号 |
A01H5/00;C07K14/415;C12N5/10;C12N15/09;C12N15/29;C12N15/82;(IPC1-7):C12N15/82;A01H1/02;C12N5/04;A01H5/10 |
主分类号 |
A01H5/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|