摘要 |
FIELD: biotechnology, microbiology, organic chemistry. ^ SUBSTANCE: invention relates to a method for preparing organic acids, in particular, citric acid. Method involves isolation of calcium citrate and acid stable amylolytic enzymes from the solution cultural fluid after fermentation with fungus Aspergillus niger. The isolation process is carried out at temperature 10-50°C and pH 3.2-5.9. Method provides increasing yield of calcium citrate and complex of acid stable amylolytic enzymes: alpha-amylase and glucoamylase. ^ EFFECT: improved preparing method. ^ 1 tbl, 6 ex |