发明名称 |
Verfahren zur Herstellung von trans-4-Hydroxy-L-Prolin |
摘要 |
Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel transformant containing a gene, coding for L-proline-4-hydroxylase and having a reinforced L-proline biosynthesis activity. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity. |
申请公布号 |
DE69732327(T2) |
申请公布日期 |
2005.10.06 |
申请号 |
DE1997632327T |
申请日期 |
1997.09.03 |
申请人 |
KYOWA HAKKO KOGYO CO., LTD. |
发明人 |
OZAKI, AKIO;SHIBASAKI, TAKESHI;MORI, HIDEO;MARUYAMA, AKIHIKO;MOTOYAMA, HIROAKI |
分类号 |
C12N15/09;C07H21/04;C12N1/21;C12N9/02;C12N15/22;C12N15/52;C12N15/53;C12P13/24;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|