PYROPHOSPHORIC ACID QUANTIFICATION METHOD, PRIMER EXTENSION DETECTION METHOD AND APPARATUS FOR PERFORMING THESE METHODS

摘要

It is intended to provide a method of quantitatively measuring pyrophosphoric acid (PPi), wherein only a small number of enzymes are needed and no strict temperature-control is required, a method of detecting the extension of a primer, and a kit and an apparatus for performing these methods. Namely, a PPi quantification method which comprises treating endogenous actinomyces H<+>-pyrophosphatase (H<+>-PPase) in a membrane with a PPi sample at an unknown concentration and then analyzing the H<+>-transportation thus induced to thereby determine the PPi concentration in the sample. A primer extension detection method which comprises treating endogenous actinomyces H<+>-PPase in a membrane with a sample of an unknown nucleic acid, which is to be examined concerning the occurrence or non-occurrence of primer extension, and then analyzing the H<+>-transportation thus induced to thereby judge whether or not primer extension has been conducted.