It is intended to provide a process for producing a dipeptide except D-alanyl-D-alanine and D-alanyl-D-serine by using an optionally processed culture of a microorganism capable of producing a protein having a D-alanine-D-alanine ligase activity as an enzyme source; and a process for producing a dipeptide containing a D-amino acid by using an optionally processed culture of a microorganism capable of producing a D-amino acid and an optionally processed culture of a microorganism capable of producing a protein having a D-alanine-D-alanine ligase activity as an enzyme source.
