USE OF CHIMERIC NUCLEASES TO STIMULATE GENE TARGETING

摘要

The present invention relates to a vector comprising (a) a nucleic acid encoding a chimeric nuclease, wherein the chimeric nuclease comprises: (i) a DNA binding domain comprising a zinc finger domain; (ii) a Fok I cleavage domain; (iii) a nuclear localization signal (NLS); and (b) a nucleic acid comprising a repair substrate that comprises: (i) a nucleic acid sequence that is substantially identical to a region flanking a target sequence in chromosomal DNA; and (ii) a nucleic acid sequence which replaces the target sequence upon recombination between the repair substrate and the target sequence.