发明名称 |
Screen for CDC7 inhibitors |
摘要 |
Disclosed is a yeast cell whose genetic complement includes an inactive allele of the yeast CDC7 gene, a first nucleic acid that encodes a mammalian Cdc7 protein, and a second nucleic acid that encodes a mammalian Dbf4 protein. The yeast cell is dependent on the mammalian Cdc7 and Dbf4 proteins for viability. The yeast cell can be used to identify potential anti-proliferative agents by virtue of their inhibition of the mammalian Cdc7 and Dbf4 proteins. In some embodiments, a control yeast cell which does not depend on Cdc7 for viability is used in a secondary screen.
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申请公布号 |
US2005214742(A1) |
申请公布日期 |
2005.09.29 |
申请号 |
US20050129586 |
申请日期 |
2005.05.13 |
申请人 |
MILLENNIUM PHARMACEUTICALS, INC. |
发明人 |
AMIDON BENJAMIN S.;BULAWA CHRISTINE E. |
分类号 |
C07K14/47;(IPC1-7):C12Q1/00;C12N1/18 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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地址 |
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