ALTERED EXPRESSION IN FILAMENTOUS FUNGI

摘要

Agaricus bisporus serine protease is selectively expressed in the stipe, with maximum expression observed after 5 days at 18 °C, the promoter thereof being effective to permit selective expression of a heterologous protein in the fruiting body. Transformants can be selected without having to induce fruiting, by forcing expression using humic proteins. Post-transcriptional gene suppression is useful to suppress the serine protease to allow harvesting of heterologous proteins from the fruiting body without degradation by the serine protease.