| 摘要 |
The present invention relates to a novel compound comprising a detectable moiety covalently linked to a structural moiety. Upon cleavage of the covalent bond linking the two moieties, the detectable moiety becomes capable of complexing a lanthanide ion, and the lanthanide-detectable moiety complex provides a detectable signal. The structural moiety of the compound is a homo- or hetero-multimer of amino acids, nucleotides, or saccharides. A library comprising at least two member compounds with different structural moieties is also provided in this application. Further described are methods for identifying the substrate specificity of a hydrolytic enzyme by using the library of the present invention to determine the preferred structural moiety for any particular enzyme having the potential capability of cleaving the covalent bond between the detectable moiety and the structural moiety of the member compounds, as well as methods for using the novel compound of this invention for detecting in a sample the presence of a pre-determined hydrolytic enzyme, whose preferred substrate specificity is known and represented by the structural moiety of the compound.
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