发明名称 |
Method of classifying counting leucocytes |
摘要 |
There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).
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申请公布号 |
US2005202400(A1) |
申请公布日期 |
2005.09.15 |
申请号 |
US20050517422 |
申请日期 |
2005.02.28 |
申请人 |
TSUJI TOMOHIRO;MIZUKAMI TOSHIHIRO;KONISHI AYA;MORI YUSUKE;NAKAZAWA YUKIE |
发明人 |
TSUJI TOMOHIRO;MIZUKAMI TOSHIHIRO;KONISHI AYA;MORI YUSUKE;NAKAZAWA YUKIE |
分类号 |
G01N15/00;G01N15/14;G01N33/52;(IPC1-7):C12Q1/00 |
主分类号 |
G01N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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