发明名称 |
METHOD OF CRYOPRESERVATION OF ORGAN CULTURE OF THE THYROID GLAND OF NEW-BORN PIGLETS |
摘要 |
A method of cryopreservation of organ culture of the thyroid gland of new-born piglets involves incubation in a cryopreservation solution containing the medium 199 and the 7% dimexide as cryoprotector, freezing up to -196 DEGREE C, keeping in liquid nitrogen and warming up. The incubation of organ culture is carried out at 22 DEGREE C for 30 minutes, and the freezing is carried out above the mirror of liquid nitrogen with the speed of 85-100 DEGREE C/min for 3 minutes with the further submerging into the liquid nitrogen.
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申请公布号 |
UA9367(U) |
申请公布日期 |
2005.09.15 |
申请号 |
UA20050002813 |
申请日期 |
2005.03.28 |
申请人 |
INSTITUTE FOR PROBLEMS OF CRYOBIOLOGY AND CRYOMEDICINE NATIONAL ACADEMY OF SCIENCES OF UKRAINE |
发明人 |
BONDARENKO TETIANA PETRIVNA;BOZHOK HALYNA ANATOLIIVNA;ALABEDALKARIM NATALIIA MYKHAILIVNA |
分类号 |
A01N1/02;(IPC1-7):A01N1/02 |
主分类号 |
A01N1/02 |
代理机构 |
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