摘要 |
Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula (I) is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig - OH or Sig -O>-< is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs. |
申请人 |
LUMIGEN INC.;AKHAVAN-TAFTI, HASHEM;EICKHOLT, ROBERT, A.;LAUWERS, KENNETH, S.;HANDLEY, RICHARD, S. |
发明人 |
AKHAVAN-TAFTI, HASHEM;EICKHOLT, ROBERT, A.;LAUWERS, KENNETH, S.;HANDLEY, RICHARD, S. |