发明名称 |
Synthetic molecules for labeling histidine-rich proteins |
摘要 |
The invention provides Zn-chelating compounds that are molecularly engineered to bind to a specific target sequence in a protein of interest. The Zn<SUP>2+</SUP> ion is far less toxic and promiscuous than nickel and therefore provides an attractive alternative to Ni-based labeling systems. Invention Zn-chelating compounds also do not require oxidizable thiols and therefore can be used in non-reducing environments such as the surface of living cells. In addition, the target sequence is genetically encodable and requires incorporation of only a few amino acids, unlike fusions to fluorescent proteins such as GFP.
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申请公布号 |
US6933384(B2) |
申请公布日期 |
2005.08.23 |
申请号 |
US20030346658 |
申请日期 |
2003.01.16 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
TSIEN ROGER Y.;HAUSER CHRISTINA |
分类号 |
C07D405/12;(IPC1-7):C07D265/38;C07D311/80;C07D405/14 |
主分类号 |
C07D405/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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