发明名称 |
Crystallized glucocorticoid receptor ligand binding domain polypeptide and screening methods employing same |
摘要 |
A method of modifying a test nuclear receptor (NR) polypeptide is disclosed. The method provides a test NR polypeptide sequence having a characteristic that is targeted for modification; aligning the test NR polypeptide sequence with at least one reference NR polypeptide sequence for which an X-ray structure is available; building a three-dimensional model for the test NR polypeptide using the three-dimensional coordinates of the X-ray structure(s) of at least one reference polypeptide and its sequence alignment with the test NR polypeptide sequence; examining the three-dimensional model of the test NR polypeptide sequence for characteristic differences with the reference polypeptide; and mutating at least one amino acid residue in the test NR polypeptide sequence at a characteristic difference, whereby the test NR polypeptide is modified.
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申请公布号 |
US2005181362(A1) |
申请公布日期 |
2005.08.18 |
申请号 |
US20040484061 |
申请日期 |
2004.01.16 |
申请人 |
APOLITO CHRISTOPHER J.;BLEDSOE RANDY K.;LAMBERT III MILLARD H.;MCKEE DAVID D.;MONTANA VALERIE G.;PEARCE KENNETH H.;STANLEY THOMAS B.;XU HUAQIANG E.;DELVES CHRISTOPHER J. |
发明人 |
APOLITO CHRISTOPHER J.;BLEDSOE RANDY K.;LAMBERT III MILLARD H.;MCKEE DAVID D.;MONTANA VALERIE G.;PEARCE KENNETH H.;STANLEY THOMAS B.;XU HUAQIANG E.;DELVES CHRISTOPHER J. |
分类号 |
A61P17/06;A61P35/02;A61P35/04;C07K14/72;C12N9/22;C12Q1/68;G01N33/48;G01N33/50;G01N33/53;G01N33/74;G06F19/16;G06F19/22;(IPC1-7):C12Q1/68;G06F19/00 |
主分类号 |
A61P17/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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