POLYMERASE-BASED PROTOCOLS FOR THE INTRODUCTION OF COMBINATORIAL DELETIONS AND INSERTIONS

摘要

The present invention relates to improved methods of introducing combinatorial mutations in a region of DNA without the need for subcloning. The invention comprises polymerase-based, mutagenesis methods which may be adapted for use with commercially available mutagenesis kits to generate combinatorial mutations of a region of DNA in a quick, efficient and cost-effective manner. Major applications of this method include vaccine production, directed evolution and other areas that benefit from the development of diversity.