TAQ DNA-POLYMERASE MIT EINER AMINOSÄURESUBSTITUTION IN POSITION E681 UND ENTSPRECHENDE HOMOLOGE MIT EINER VERBESSERTEN SALZTOLERANZ, NÜTZLICH FÜR DIE SEQUENZIERUNG SCHWIERIGER TEMPLATESEQUENZEN MITTELS GELADENEN ODER UNGELADENEN TERMINATOREN

摘要

Thermostable DNA polymerases having an E410R substitution which result in a substantial improvement of signal uniformity compared to Taq DELTA 271/F272M/F667Y DNA polymerase. The instant DNA polymerases possess improved salt tolerance and have been shown to modulate the incorporation of terminators having a net positive or a net negative charge during the sequencing reaction.