FREQUENCY DOMAIN LUMINESCENCE INSTRUMENTATION

摘要

Instrumentation for measuring luminescence phase lag to quantitate an analyte concentration is corrected to eliminate or reduce extraneous phase lag noise. A calibration factor is determined in steps that are interspersed between quantitative measurements. An optical pathway is provided to accomplish the calibration by the provision of a second optical source (302) that emits in the luminescence emission band of a luminescent material. The calibration factor may be subtracted from measurement of the quantification phase lag to correct for extraneous phase lag.