СИНТЕТИЧЕСКИЕ ОЛИГОНУКЛЕОТИДНЫЕ ПРАЙМЕРЫ И СПОСОБ ВЫЯВЛЕНИЯ ДНК ВИРУСА ИНФЕКЦИОННОГО РИНОТРАХЕИТА КРУПНОГО РОГАТОГО СКОТА С ПОМОЩЬЮ СПЕЦИФИЧЕСКИХ ОЛИГОНУКЛЕОТИДНЫХ ПРАЙМЕРОВ В ПОЛИМЕРАЗНОЙ ЦЕПНОЙ РЕАКЦИИ (ПЦР)

摘要

FIELD: biotechnology, genetic engineering, molecular biology, veterinary science. ^ SUBSTANCE: invention proposes synthetic primers that are complementary to highly conservative region in cattle infectious rhinotracheitis virus genome in region of thymidine kinase (tk) gene. Proposed primers are used for detecting cattle infectious rhinotracheitis virus DNA using polymerase chain (PCR) reaction method. Proposed method involves carrying out amplification by PCR method. For carrying out PCR method involves using synthetic primers that are complementary to highly conservative region in cattle infectious rhinotracheitis virus genome in region of thymidine kinase gene (tk). Results of the reaction are evaluated without preliminary restriction of the prepared product. Result is considered to be positive if PCR product corresponds to fragment size 298 base pairs. Method can be used in veterinary virology for detection of infectious diseases in agricultural animals, in particular, for detection of infectious rhinotracheitis in cattle. ^ EFFECT: improved detecting method. ^ 2 tbl, 7 ex