PRIMER FOR NUCLEIC ACID AMPLIFICATION, PRIMER SET FOR NUCLEIC ACID AMPLIFICATION AND METHOD OF CANCER SCREENING THEREWITH

摘要

A method of detecting any mutation at codon 12 and codon 13 of KRAS gene with high reproducibility and with high accuracy conveniently by one operation; and, for use in this detection method, a primer for nucleic acid amplification, primer set for nucleic acid amplification and detection reagent kit for mutation at codon 12 and codon 13 of KRAS gene. There is provided amplification of KRAS gene performed with the use of a primer set for nucleic acid amplification that acts on KRAS gene and contains a primer for nucleic acid amplification having a mismatch introduced in its base sequence.