| 摘要 |
The invention relates to a method for providing a defined fluid flow, especially for use in liquid chromatography. According to the method, a constant total flow (f0) is subdivided into an internal excess flow (fie) in an excess branch and into an internal working flow (fiw) in a working branch . The ratio of subdivision of the internal working flow (fiw) and the internal excess flow (fie) depends on the reverse ratio of a fluidic resistance provided in the working branch and a fluidic resistance in the excess branch . The excess branch and the working branch are interlinked at the respective outputs of the two fluidic outputs of the fluidic resistances by a cross- branch. The equalizing flow occurring between the outputs of the fluidic resistances is measured by means of a flow sensor. A desired, external worki ng flow in the further course of the working branch can be fed to a working device, for example a chromatography column mounted downstream of the device . Further down the excess branch a variable fluidic resistance device is arranged. The resistance value of the variable fluidic resistance device is controlled, thereby controlling the equalizing flow in such a manner that th e equalizing flow, preferably in the temporal mean, is substantially zero or equals a defined offset value whoseamount is small compared to the internal working flow (fiw). The invention also relates to a device for carrying out the inventive method. |
