发明名称 |
METHOD FOR CHARACTERISING POLYPEPTIDES BY MALDI-TOF MAS SPECTROMETRY |
摘要 |
Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte; wherein the light absorbing label comprises a fluorophore moiety, and wherein prior to detecting by mass spectrometry, the analyte is selected for detection on the basis of its fluorophore moiety. |
申请公布号 |
WO2005012914(A3) |
申请公布日期 |
2005.06.30 |
申请号 |
WO2004GB03139 |
申请日期 |
2004.07.22 |
申请人 |
XZILLION GMBH & CO. KG;PROTEOME SCIENCES PLC;SCHAFER, JURGEN;HAMON, CHRISTIAN;SCHWARZ, JOSEF;PEARCE, CHRISTOPHER |
发明人 |
SCHAFER, JURGEN;HAMON, CHRISTIAN;SCHWARZ, JOSEF;PEARCE, CHRISTOPHER |
分类号 |
C07D403/14;C07D413/14;C07K1/13;G01N33/533;G01N33/68 |
主分类号 |
C07D403/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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