A vaccine-induced hepatitis B viral strain and uses thereof

摘要

Disclosed is a method of obtaining antibodies to a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, which differ from the amino acid sequence of a major surface antigen of a wildtype hepatitis B virus in that the amino acid at position number 145 of such polypeptide is an arginine, rather than a glycine, and not to the major surface antigen of a wildtype hepatitis B virus, comprising: (a) obtaining the polypeptide in a purified form; (b) immunizing a nonùhuman organism capable of producing antibodies against the purified polypeptide; (c) collecting the produced antibodies; (d) combining the produced antibodies and the purified polypeptide under conditions to form a complex; and (e) determining which produced antibodies form a complex with the purified polypeptide so as to obtain antibodies to the polypeptide. Such polypeptides are useful for determining whether a subject is infected with a strain of hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-æSÆ-145 Singapore strain (glycine to arginine). (62) Divided out of 508892