| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for determining a whale species by a SINE (short interspersed element) method. <P>SOLUTION: The method for determining the whale species comprises making each genome DNA library from one or more whale species to be determined and isolating a clone containing an orthologues gene locus into which a SINE family or subfamily is specifically inserted in at least one kind of species among the whale species, respectively. In the gene locus, the gene locus sequence in each of the whale species is amplified by PCR (polymerase chain reaction) by using a set of a PCR primer annealing flanking sequences positioned at both ends of the SINE family or subfamily. The obtained PCR product is subjected to gel electrophoresis and the whale species is determined by presence of a band exhibiting the existence of the gene locus into which the SINE family or subfamily is inserted. The gene locus sequence and the primer sequence useful in the method are obtained. <P>COPYRIGHT: (C)2005,JPO&NCIPI |
