| 摘要 |
FIELD: biochemistry, in particular production of immobilized enzymes useful on chemistry, biochemistry, medicine, histology, microbiology, ecology and agriculture for bioluminescence analysis. ^ SUBSTANCE: target reagent is obtained by production of 3-5 % gel by boiling of starch suspension in phosphate buffer, gel cooling to 24-30°C, mixing of buffer solution of luminescent bacterium bienzyme system NADH:FMH-oxydoreductase-lucirerase with starch gel, dosage on lavsan film and drying at 4-10°C. According the invention components are introduced in the next order: trimyristin aldehyde, nicotinamidadeninedinucleotide, bienzyme system NADH:FMH-oxydoreductase-lucirerase, and flavin mononucleotide. Substrate solution of nicotinamidadenine dinucleotide, flavin mononucleotide, and trimyristin aldehyde are prepared in phosphate buffer with pH 6.8-7.0. ^ EFFECT: reagent of improved quality due to decreased quantity of reaction mixture components (from four to one), reduced analysis period by two times and increased measurement accuracy. ^ 2 cl, 1 dwg, 4 ex |
