A method is provided which allows high yields of acylated insulin. The method comprises expressing a singe-chain insulin precursor, preferably in yeast, cleaving the single-chain insulin precursor with a suitable protease which will open the peptide bond between the C-terminal amino group in the B-chain and a connecting peptide connecting the B chain with the A-chain, acylating the two-chain insulin intermediate in the e-amino group in LysB29 and subjecting the acylated two-chain insulin intermediate to a proteolytic enzyme which will cleave of the N-terminal extension on the B- and A-chains on the precursor molecule.
申请公布号
WO2005047508(A1)
申请公布日期
2005.05.26
申请号
WO2004DK00782
申请日期
2004.11.11
申请人
NOVO NORDISK A/S;KJELDSEN, THOMAS, BOERGLUM;MARKUSSEN, JAN