发明名称 |
Modular isotope coding approach to proteomic analysis |
摘要 |
The present invention provides methods for analyzing a peptide or peptides of interest in a protein sample using a combination of a relatively generic isotope tag with a decoupled selection process, allowing simplified customization of the application with a single reagent. These methods comprise providing a first and a second protein sample; labeling the first protein sample with a first Universal Peptide Isotope Tag (U-PIT) reagent and the second protein sample with a second U-PIT reagent; separating the peptide of interest from the combined first and second protein samples; and determining the relative amount of the first U-PIT reagent and the second U-PIT reagent bound to the peptide or peptides of interest. The U-PIT label of the present inventive methods has the following general formula <?in-line-formulae description="In-line Formulae" end="lead"?>A-B-C <?in-line-formulae description="In-line Formulae" end="tail"?> wherein A is a nucleophilic reactive group, B is a detectable moiety that can be isotopically labeled, and C is a charge replacement group.
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申请公布号 |
US2005100956(A1) |
申请公布日期 |
2005.05.12 |
申请号 |
US20020318475 |
申请日期 |
2002.12.13 |
申请人 |
APFFEL JAMES A.JR.;ROBOTTI KARLA M. |
发明人 |
APFFEL JAMES A.JR.;ROBOTTI KARLA M. |
分类号 |
G01N33/68;(IPC1-7):G01N33/53;C07K16/46 |
主分类号 |
G01N33/68 |
代理机构 |
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