发明名称 |
PROCESS FOR PURIFYING PROTEINS IN A HYDROPHOBIC INTERACTION CHROMATOGRAPHY FLOW-THROUGH FRACTION |
摘要 |
<p>The present invention is a process for separating a target protein (such as a recombinant protein produced in a cell culture) from a mixture containing the target protein and contaminants (such as cell culture contaminants), by contacting the mixture with a hydrophobic adsorbent comprising branched hydrocarbon functional groups in an aqueous salt solution and collecting the unbound flow-through fraction containing the target protein. In one embodiment, the hydrophobic adsorbent may be a branched alkyl functional group. In another embodiment, the branched alkyl functional group has from 3 to 8 carbon atoms. In another embodiment, the branched alkyl functional group is a tertiary carbon atom, such as tert-butyl.</p> |
申请公布号 |
WO2005042569(A1) |
申请公布日期 |
2005.05.12 |
申请号 |
WO2004US35007 |
申请日期 |
2004.10.22 |
申请人 |
AMGEN, INC.;SHUKLA, ABHINAV, A.;GHOSE, SANCHAYITA |
发明人 |
SHUKLA, ABHINAV, A.;GHOSE, SANCHAYITA |
分类号 |
C07K1/10;C07K1/20;C07K14/00;C12N9/00;C12P21/06;(IPC1-7):C07K14/00 |
主分类号 |
C07K1/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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