发明名称 |
Hybridization-based fluorescence assay |
摘要 |
The invention provides a method for detecting the presence or absence of an oligonucleotide in a sample. In the method according to the invention, a sample is obtained that may contain a target oligonucleotide. An oligonucleotide that is complementary to the target oligonucleotide and a fluorophore are added to the sample to form a mixture. The mixture is then incubated under hybridization conditions to allow binding of the complementary oligonucleotide to the target oligonucleotide to form a duplex. The fluorophore binds to the duplex; and fluorescence is then detected by standard procedures. The detection of fluorescence is indicative of the presence of the target oligonucleotide in the sample.
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申请公布号 |
US2005089915(A1) |
申请公布日期 |
2005.04.28 |
申请号 |
US20040974129 |
申请日期 |
2004.10.27 |
申请人 |
HYBRIDON, INC. |
发明人 |
KANDIMALLA EKAMBAR R.;AGRAWAL SUDHIR |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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