发明名称 |
DIGITALE AMPLIFIZIERUNG |
摘要 |
<p>The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose. Single molecules can be isolated by dilution and individually amplified; each product is then separately analyzed for the presence of pre-defined mutations. The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA sample.</p> |
申请公布号 |
DE60018616(D1) |
申请公布日期 |
2005.04.14 |
申请号 |
DE2000618616 |
申请日期 |
2000.07.31 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY, BALTIMORE |
发明人 |
KINZLER, W.;VOGELSTEIN, BERT |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|