DNA sequence encoding A. thaliana protein exhibiting delta-5,7 sterol, delta-7 reductase activity, the protein delta-7-Red, A. thaliana protein, process of their preparation, strain structures of transformed yeast and application thereof

摘要

In the present invention, there is disclosed a nucleic acid sequence containing a sequence coding for organism A. thaliana protein having a delta-5,7 sterol, delta-7 reductase activity (sequence SEQ ID No. 1), a primer for amplification, A. thaliana protein, antibodies, cloning method, reduction process of sterols that are unsaturated in position C-7, process for preparing pregnenolone, wherein the host cell is yeast, transformed strain of yeast, designated with EC01/pCD63 under access number CNCM I-1538 and application thereof.