| 摘要 |
FIELD: medicine. ^ SUBSTANCE: invention refers to medicine and describes method of glycosaminoglycan release from mineralised connective tissue, containing enzymatic hydrolysis, deproteinisation, deposition, and characterised by the fact that demineralisation is performed within 20 h using 0.5 H HC1, enzymatic hydrolysis is performed within 18 h using pepsin enzyme, deproteinisation is performed by adding of ammonium sulphate to 75% saturation, glycosaminoglycans are deposited with 4% potassium acetate in 96% ethanol, re-deposited with ethanol and lyophilised. Glycosaminoglycans are exposed to chromatographic separation using ""-sephadex A-25, and lyophilised. This method is easy-to-use, realised in laboratory environment of patient care institutions, and time-saving. ^ EFFECT: easy and saving method of glycosaminoglycan release from mineralised connective tissue. ^ 1 ex, 2 dwg |
