发明名称 |
Gfp-transfected clon pig, gt knock-out clon pig and methods for productions thereof |
摘要 |
Disclosed are a cloned pig expressing green fluorescent protein (GFP) and a cloned pig having a 1,3-galactosyltransferase (GT) gene knocked out. Also, the present invention discloses methods of producing such cloned pigs, comprising the steps of establishing a somatic cell line; preparing a GFP-transfected or GT gene knock-out nuclear donor cell; producing a transgenic nuclear transfer embryo using the nuclear donor cell and a recipient oocyte; and transplanting the transgenic nuclear transfer embryo into a surrogate mother pig. The cloned pig expressing GFP of the present invention is useful for large-scale production of an animal disease model, and the GT gene knock-out cloned pig can be used as a organ donor allowing xenotransplantation in humans without hyperacute immune rejection.
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申请公布号 |
US2005076399(A1) |
申请公布日期 |
2005.04.07 |
申请号 |
US20040500748 |
申请日期 |
2004.11.30 |
申请人 |
LEE SO H.;HWANG WOO S.;LEE BYEONG C.;KANG SUNG K.;HAN JEK Y.;LIM JEONG M.;LEE CHANG K.;LEE EUN S.;JEUNG EUI B.;CHO JONG K.;KIM DAE Y.;HYUN SANG H.;LEE GAB S.;KIM HYE S.;LEE SUNG C.;YEOM SU C. |
发明人 |
LEE SO H.;HWANG WOO S.;LEE BYEONG C.;KANG SUNG K.;HAN JEK Y.;LIM JEONG M.;LEE CHANG K.;LEE EUN S.;JEUNG EUI B.;CHO JONG K.;KIM DAE Y.;HYUN SANG H.;LEE GAB S.;KIM HYE S.;LEE SUNG C.;YEOM SU C. |
分类号 |
A01K67/02;A01K67/027;C12N5/16;C12N15/09;C12N15/877;(IPC1-7):A01K67/027 |
主分类号 |
A01K67/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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