EFFICIENT METHOD OF PREPARING DNA INVERTED REPEAT STRUCTURE

摘要

In the conventional technology of preparing a chimera gene having an inverted repeat structure of target sequence, a target sequence is inserted in two sites on a vector in the sense direction and the antisense direction, so that independent restriction enzyme recognizing sequences must be provided at not only insertion sites on the vector but also both ends of the target sequence, resulting in complications. In the invention, a chimera gene having an inverted repeat structure is prepared by providing a cassette construct having an arbitrary adaptor sequence and an inverted adaptor sequence arranged with an arbitrary spacer sequence interposed therebetween or preparing a plasmid vector having such a cassette construct incorporated therein; bonding a target sequence to one or both ends of the cassette construct, or inserting a target sequence in one end of the cassette construct on the plasmid vector; and thereafter carrying out PCR.