| 摘要 |
<p>The invention relates to a polarisation fluorometer and to a method for calibrating such a fluorometer. The fluorometer comprises an excitation channel with an excitation polariser (3H, 3V) for generating light polarised in a first plane or in a second plane to be conducted as a excitation light to a sample, an emission channel for conducting emission light from the sample, in which emission channel there is an emission polarisation filter (5H), an excitation detector (8), to which light polarised by the excitation polariser can be conducted from the excitation channel, and a reference detector (10), to which light polarised by the excitation polariser can be conducted from the excitation channel before being conducted to the sample or to the excitation detector. The reference detector can be calibrated by means of the excitation detector so that correction measurements can be carried out in real time during sample measurement. The fluorometer can be used especially for fluorescence spectra measurements.</p> |
