Transfection high-voltage controller

摘要

A method for operating a transfection system adapted to deliver a prespecified high-voltage current to a chemical solution, comprising the steps of: asserting a trigger signal when power is removed from the transfection system; triggering a crowbar circuit coupled to a charge storage device that provides the high-voltage current when said trigger signal is asserted; and discharging said charge storage device through said crowbar circuit when said crowbar circuit is activated. An apparatus for directing a first predetermined current profile at a second predetermined high voltage level to a chemical solution is also disclosed, along with a hi-joule switch and a capacitor expansion module.