Method to enable assessment of growth and death of micro-organisms

摘要

The invention is a method of assessing growth and death rates of micro-organisms within a time period environment of interest. Two reporter genes coding for luminescent and/or fluorescent products introduced into the micro-organism produce at least two products which luminesce or fluoresce. They include, either an essentially stable product produced in an essentially known proportion to the total amount of cells of the micro-organism that are or have been alive within a time period, a product present in an essentially known proportion to the amount of cells alive at any moment within the time period, or an essentially stable product produced in an essentially known proportion to the total amount of cells of the micro-organism that have died within the time period. The micro-organism is incubated and luminescence and/or fluorescence is detected after at time period. Finally, the growth and death rates of the micro-organism are assessed.