发明名称 Automated protein purification the multiwell format by vacuum filtration
摘要 The invention relates to a process and a reagent kit for obtaining clear solutions containing cell contents from biological samples in a high throughput process, comprising the steps of preparing a plurality of protein-containing solutions which contain insoluble ingredients, in separate chambers of a multi-chamber filtration unit, eliminating insoluble ingredients by filtering the solutions through the multi-chamber filtration unit with the application of a pressure differential, while preventing cross contamination between adjacent chambers by chemical and/or mechanical means, and collecting the individual filtrates separately in collecting containers, as well as a process for recovering cell contents from the clear solutions obtained. The invention further relates to the use of an apparatus for recovering clear protein-containing solutions from biological samples.
申请公布号 US6864100(B1) 申请公布日期 2005.03.08
申请号 US20020049003 申请日期 2002.02.04
申请人 QIAGEN GMBH 发明人 RIBBE JOACHIM;SCHAEFER FRANK;STEINERT KERSTIN;LUBENOW HELGE
分类号 B01J20/281;B01D19/04;B01L3/00;C07K1/14;C07K1/34;C12M1/32;C12N9/10;C12N15/10;C12Q1/02;G01N1/34;G01N30/88;(IPC1-7):G01N1/34 主分类号 B01J20/281
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