METHOD OF BREEDING LIPID-PRODUCING FUNGUS

摘要

It is intended to provide a breeding method whereby a lipid-producing fungus belonging to the genus Mortierella can be effectively and efficiently bred without resort to antibiotics. Transformation is carried out with the use of an auxotroph (for example, uracil auxotroph) of a fungus belonging to the genus Mortierella as a host. More specifically speaking, a gene compensating for the auxotrophy, which is employed as a marker gene, is transferred into the host (the gene transfer step). Next, a transformant is selected by using the recovery of the auxotrophy as the marker (the selection step). As an example of the lipid-producing fungus as described above, M. aplina may be cited.