<p>The present invention combines the use of template switching with nucleic acid amplification - such as PCR - to generate amplification products representative of an entire RNA population. An RNA polymerase promoter sequence allows transcription-based amplification to be performed on the derived amplification products such that antisense amplified RNA (aRNA) or complementary RNA (cRNA) is produced for subsequent downstream applications.</p>
申请公布号
WO2005019452(A1)
申请公布日期
2005.03.03
申请号
WO2004GB03486
申请日期
2004.08.12
申请人
ASTRAZENECA AB;ASTRAZENECA UK LIMITED;GRAHAM, ALEXANDER;CASSIDY, ANDREW