发明名称 |
Method for the detection of nucleic acid molecules |
摘要 |
There is provided a method of simultaneously detecting at least two mutually different nucleic acid molecules in a sample, wherein in a first step a multiplex-PCR and in a second step a hybridizing reaction is carried out with probes immobilized on a microarray, whereupon the hybridized PCR products are detected and optionally quantitated, wherein the probes employed for the hybridizing reaction which in each case will hybridize specifically with the mutually different nucleic acid molecules have melting temperatures which differ from each other by 2° C. at the most, preferably by 1° C. at the most. |
申请公布号 |
CZ20032665(A3) |
申请公布日期 |
2005.02.16 |
申请号 |
CZ20030002665 |
申请日期 |
2002.03.01 |
申请人 |
AUSTRIAN RESEARCH CENTERS GMBH - ARC |
发明人 |
SCHMIDT WOLFGANG;MUNDLEIN AXEL;HUBER MARTIN;KROATH HANS |
分类号 |
G01N33/53;C12M1/00;C12N15/09;C12Q1/68;G01N33/566;G01N37/00 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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