Regeneration of hydrolysis sensitive adsorbent matrices

摘要

The present invention relates to the regeneration of adsorbent matrices that are used for the purification of substances from aqueous liquid samples derived from biological material. The method is adapted to adsorbent matrices that are sensitive to hydrolysis either because of a sensitive base matrix or a sensitive ligand including i.e. matrices having a proteineous ligands, and also a sensitive spacer and is characterized in contacting said adsorbent matrix with a first regeneration solution, which is aqueous and contains a water-miscible organic solvent and has a pH value≤4, preferably≤3 (acid solution) and subsequently contacting said adsorbent matrix with a second regeneration solution, which is aqueous and has a pH value≥10 but≤13 (alkaline solution).