NOVEL SPHINOGMYELINASE, THE ANTIBODY AGAINST IT, THE ANTISENSE AND THE PREPARATION METHOD THEREOF

摘要

The present invention relates to a novel neutral sphingomyelinase, its antibody, its antisense and the preparation method therof. The purified neutral sphingomyelinase enzyme, designated as N-SMase ¥åderived from mammalian brain, is HSP60 protein which has 60kDa of M.W. determined by SDS-PAGE analysis and has Mg+-dependent enzyme activity. The N-SMase ¥åenzyme which produces ceramide can induce apoptosis therefore a composition comprising the same can be useful for preventing and treating cancer caused by abnormal cell proliferation. Also, the antibody against inventive N-SMase ¥å enzyme inhibits the enzyme activity and the antisense against inventive N-SMase ¥åenzyme suppress the enzyme expression and activation, thereby they can be used for the manufacture of therapeutics for treating apoptosis, aging and inflammation-related diseases caused by ceramide.