METHOD OF EFFICIENTLY CONSTRUCTING GROWTH REGULATORY RECOMBINANT ADENOVIRUS VECTOR AND KIT FOR THE CONSTRUCTION THEREOF

摘要

A method of constructing a growth regulatory recombinant adenovirus vector plasmid comprising: preparing a vector plasmid having a growth regulatory unit, which has an E1A region, a protein-coding region of an E1B region, a poly A signal sequence and a recombinase recognition sequence in this order from the upstream, lacks an endogenous promoter in the E1A region and an endogenous promoter controlling the expression of a gene encoding the protein in the protein-coding region of the E1B region, and has restriction enzyme recognition sequences inserted into the deletion sites; then transferring promoters expressed specifically in a target tissue into the restriction enzyme recognition sequences to thereby give a growth regulatory vector plasmid; and then integrating this growth regulatory vector plasmid into a vector plasmid having an adenovirus genome constructed by deleting the E1 region to thereby give a growth regulatory adenovirus vector plasmid.