| 摘要 |
PURPOSE: A method for identification of a Bifidobacterium sp. strain is provided, thereby rapidly and accurately identifying the Bifidobacterium sp. strain using Bifidobacterium sp. specific and common primers. CONSTITUTION: A Bifidobacterium sp. specific primer has the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:8. A Bifidobacterium sp. common primer has the nucleotide sequence selected from SEQ ID NO:9 and SEQ ID NO:10. The method for identification of a Bifidobacterium sp. strain comprises the steps of: (1) PCR amplifying a test chromosomal DNA as a template using one Bifidobacterium sp. specific primer selected from SEQ ID NO:1 to SEQ ID NO:8 and one Bifidobacterium sp. common primer selected from SEQ ID NO:9 to SEQ ID NO:10; and (2) determining whether the PCR product is amplified and the size of the PCR product, wherein the Bifidobacterium sp is one or more selected from B. adolescentis, B. animalis, B. bifidum, B. breve, B. infantis, B. lactis, B. longum and B. pseudolongum.
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