СПОСОБ ПОЛУЧЕНИЯ ДИАГНОСТИКУМА ДЛЯ ВЫЯВЛЕНИЯ ЭНДОТОКСИНА

摘要

FIELD: medicine, veterinary and food processing industry. ^ SUBSTANCE: claimed method includes manufacturing of corn-shell-type latex, wherein corn represents polystyrene latex and shell represents styrene-zinc methacrylate copolymer latex in repeating unit mass ratio of 1:0.8-1:0.4. Then latex is sensitized with monoclonal antibodies of lgG2a and lgG2b fractions in 0.01 M glycine buffer at pH 8.2-8.6 without preliminary conditioning followed by incubation primarily at 36-38°C for 120-180 min and further at 2-8°C for 1-20 hours. Obtained diagnosticum has specificity of 99±0.89, sensibility up to 10 pg/ml and makes it possible to obtain investigation results for 10 min or less. ^ EFFECT: accelerated method for diagnosticum production. ^ 6 tbl