| 摘要 |
The present invention relates to a fusion protein which comprises an epitope tag, a protein translocation domain, a ubiquitin domain, and a pathogen derived immunogen. The epitope tag, the protein translocation domain, the ubiquitin domain, and the pathogen derived immunogen are directly or indirectly coupled together. Alternatively, the present invention is directed to a nucleic acid construct encoding a fusion protein where the nucleic acid construct comprises a nucleic acid molecule encoding an epitope tag, a nucleic acid molecule encoding a protein translocation domain, a nucleic acid molecule encoding a ubiquitin domain, and a nucleic acid molecule encoding a pathogen derived immunogen. The nucleic acid molecules encoding the epitope tag, the protein translocation domain, the ubiquitin domain, and the pathogen derived immunogen are directly or indirectly coupled together. Methods of vaccinating a subject against a pathogen by administering the fusion protein or the nucleic acid construct to the subject under conditions effective to vaccinate the subject against the pathogen are also disclosed. |
